amino acids Search Results


bpa uptake assay  (Dojindo Labs)
93
Dojindo Labs bpa uptake assay
(A) Schematic depiction of the LAT1-dependent uptake assay for import of the amino acid analog π-Boronophenylalanine <t>(L-BPA).</t> Cell were treated with L-BPA for 5min (1) after which cells were extensively washed, followed by the addition of a specific membrane permeable probe for additional 5 min (2), which emits fluorescent signal when bound to L-BPA(3). (B) Graph shows L- <t>BPA</t> <t>uptake</t> by Myd88 KO iBMDMs infected with the indicated Lp strains at MOI=20 or left uninfected (UN). In some control conditions, the LAT1 inhibitor 2-Aminobicyclo [2.2.1] heptane-2-carboxylic acid (BCH) at 1mM was added 10 min prior to and for the duration of the L-BPA uptake assay. BPA uptake lasted 5min and was measured at 4 hpi, after which fluorescence output was measured, the cells were lysed and total protein content was measured. Mean fluorescence data was normalized to the total protein content. Averages ± SEM of data from three biological repeats are shown for each condition. Statistical analysis was completed via one-way ANOVA Kruskal-Wallis test with Dunn’s multiple comparison and the respective p-values among the conditions compared are indicated.
Bpa Uptake Assay, supplied by Dojindo Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/amino+acids/bio_rxiv__2025__02__20__639245-305-0-3?v=Dojindo+Labs
Average 93 stars, based on 1 article reviews
bpa uptake assay - by Bioz Stars, 2026-08
93/100 stars
  Buy from Supplier
advancebio aaa standards  (Agilent technologies)
95
Agilent technologies advancebio aaa standards
(A) Schematic depiction of the LAT1-dependent uptake assay for import of the amino acid analog π-Boronophenylalanine <t>(L-BPA).</t> Cell were treated with L-BPA for 5min (1) after which cells were extensively washed, followed by the addition of a specific membrane permeable probe for additional 5 min (2), which emits fluorescent signal when bound to L-BPA(3). (B) Graph shows L- <t>BPA</t> <t>uptake</t> by Myd88 KO iBMDMs infected with the indicated Lp strains at MOI=20 or left uninfected (UN). In some control conditions, the LAT1 inhibitor 2-Aminobicyclo [2.2.1] heptane-2-carboxylic acid (BCH) at 1mM was added 10 min prior to and for the duration of the L-BPA uptake assay. BPA uptake lasted 5min and was measured at 4 hpi, after which fluorescence output was measured, the cells were lysed and total protein content was measured. Mean fluorescence data was normalized to the total protein content. Averages ± SEM of data from three biological repeats are shown for each condition. Statistical analysis was completed via one-way ANOVA Kruskal-Wallis test with Dunn’s multiple comparison and the respective p-values among the conditions compared are indicated.
Advancebio Aaa Standards, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/amino+acids/10__3389_slash_fsufs__2021__620426-65-0-9?v=Agilent+technologies
Average 95 stars, based on 1 article reviews
advancebio aaa standards - by Bioz Stars, 2026-08
95/100 stars
  Buy from Supplier
amino acid feeding experiment  (Macklin Inc)
86
Macklin Inc amino acid feeding experiment
(A) Schematic depiction of the LAT1-dependent uptake assay for import of the amino acid analog π-Boronophenylalanine <t>(L-BPA).</t> Cell were treated with L-BPA for 5min (1) after which cells were extensively washed, followed by the addition of a specific membrane permeable probe for additional 5 min (2), which emits fluorescent signal when bound to L-BPA(3). (B) Graph shows L- <t>BPA</t> <t>uptake</t> by Myd88 KO iBMDMs infected with the indicated Lp strains at MOI=20 or left uninfected (UN). In some control conditions, the LAT1 inhibitor 2-Aminobicyclo [2.2.1] heptane-2-carboxylic acid (BCH) at 1mM was added 10 min prior to and for the duration of the L-BPA uptake assay. BPA uptake lasted 5min and was measured at 4 hpi, after which fluorescence output was measured, the cells were lysed and total protein content was measured. Mean fluorescence data was normalized to the total protein content. Averages ± SEM of data from three biological repeats are shown for each condition. Statistical analysis was completed via one-way ANOVA Kruskal-Wallis test with Dunn’s multiple comparison and the respective p-values among the conditions compared are indicated.
Amino Acid Feeding Experiment, supplied by Macklin Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/amino+acids/pm41114871-101-2-7?v=Macklin+Inc
Average 86 stars, based on 1 article reviews
amino acid feeding experiment - by Bioz Stars, 2026-08
86/100 stars
  Buy from Supplier
non essential 603 amino acids neaa  (Valiant Co Ltd)
95
Valiant Co Ltd non essential 603 amino acids neaa
(A) Schematic depiction of the LAT1-dependent uptake assay for import of the amino acid analog π-Boronophenylalanine <t>(L-BPA).</t> Cell were treated with L-BPA for 5min (1) after which cells were extensively washed, followed by the addition of a specific membrane permeable probe for additional 5 min (2), which emits fluorescent signal when bound to L-BPA(3). (B) Graph shows L- <t>BPA</t> <t>uptake</t> by Myd88 KO iBMDMs infected with the indicated Lp strains at MOI=20 or left uninfected (UN). In some control conditions, the LAT1 inhibitor 2-Aminobicyclo [2.2.1] heptane-2-carboxylic acid (BCH) at 1mM was added 10 min prior to and for the duration of the L-BPA uptake assay. BPA uptake lasted 5min and was measured at 4 hpi, after which fluorescence output was measured, the cells were lysed and total protein content was measured. Mean fluorescence data was normalized to the total protein content. Averages ± SEM of data from three biological repeats are shown for each condition. Statistical analysis was completed via one-way ANOVA Kruskal-Wallis test with Dunn’s multiple comparison and the respective p-values among the conditions compared are indicated.
Non Essential 603 Amino Acids Neaa, supplied by Valiant Co Ltd, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/amino+acids/10__1016_slash_j__isci__2026__115242-325-35-40?v=Valiant+Co+Ltd
Average 95 stars, based on 1 article reviews
non essential 603 amino acids neaa - by Bioz Stars, 2026-08
95/100 stars
  Buy from Supplier
nα fmoc nω  (Chem Impex International)
95
Chem Impex International nα fmoc nω
(A) Schematic depiction of the LAT1-dependent uptake assay for import of the amino acid analog π-Boronophenylalanine <t>(L-BPA).</t> Cell were treated with L-BPA for 5min (1) after which cells were extensively washed, followed by the addition of a specific membrane permeable probe for additional 5 min (2), which emits fluorescent signal when bound to L-BPA(3). (B) Graph shows L- <t>BPA</t> <t>uptake</t> by Myd88 KO iBMDMs infected with the indicated Lp strains at MOI=20 or left uninfected (UN). In some control conditions, the LAT1 inhibitor 2-Aminobicyclo [2.2.1] heptane-2-carboxylic acid (BCH) at 1mM was added 10 min prior to and for the duration of the L-BPA uptake assay. BPA uptake lasted 5min and was measured at 4 hpi, after which fluorescence output was measured, the cells were lysed and total protein content was measured. Mean fluorescence data was normalized to the total protein content. Averages ± SEM of data from three biological repeats are shown for each condition. Statistical analysis was completed via one-way ANOVA Kruskal-Wallis test with Dunn’s multiple comparison and the respective p-values among the conditions compared are indicated.
Nα Fmoc Nω, supplied by Chem Impex International, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/amino+acids/pm36949571-43-16-14?v=Chem+Impex+International
Average 95 stars, based on 1 article reviews
nα fmoc nω - by Bioz Stars, 2026-08
95/100 stars
  Buy from Supplier
5 hydroxydiclofenac  (Toronto Research Chemicals)
92
Toronto Research Chemicals 5 hydroxydiclofenac
(A) Schematic depiction of the LAT1-dependent uptake assay for import of the amino acid analog π-Boronophenylalanine <t>(L-BPA).</t> Cell were treated with L-BPA for 5min (1) after which cells were extensively washed, followed by the addition of a specific membrane permeable probe for additional 5 min (2), which emits fluorescent signal when bound to L-BPA(3). (B) Graph shows L- <t>BPA</t> <t>uptake</t> by Myd88 KO iBMDMs infected with the indicated Lp strains at MOI=20 or left uninfected (UN). In some control conditions, the LAT1 inhibitor 2-Aminobicyclo [2.2.1] heptane-2-carboxylic acid (BCH) at 1mM was added 10 min prior to and for the duration of the L-BPA uptake assay. BPA uptake lasted 5min and was measured at 4 hpi, after which fluorescence output was measured, the cells were lysed and total protein content was measured. Mean fluorescence data was normalized to the total protein content. Averages ± SEM of data from three biological repeats are shown for each condition. Statistical analysis was completed via one-way ANOVA Kruskal-Wallis test with Dunn’s multiple comparison and the respective p-values among the conditions compared are indicated.
5 Hydroxydiclofenac, supplied by Toronto Research Chemicals, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/amino+acids/pmc05961743-93-11-12?v=Toronto+Research+Chemicals
Average 92 stars, based on 1 article reviews
5 hydroxydiclofenac - by Bioz Stars, 2026-08
92/100 stars
  Buy from Supplier
non essential amino acid solution  (Beijing Solarbio Science)
95
Beijing Solarbio Science non essential amino acid solution
(A) Schematic depiction of the LAT1-dependent uptake assay for import of the amino acid analog π-Boronophenylalanine <t>(L-BPA).</t> Cell were treated with L-BPA for 5min (1) after which cells were extensively washed, followed by the addition of a specific membrane permeable probe for additional 5 min (2), which emits fluorescent signal when bound to L-BPA(3). (B) Graph shows L- <t>BPA</t> <t>uptake</t> by Myd88 KO iBMDMs infected with the indicated Lp strains at MOI=20 or left uninfected (UN). In some control conditions, the LAT1 inhibitor 2-Aminobicyclo [2.2.1] heptane-2-carboxylic acid (BCH) at 1mM was added 10 min prior to and for the duration of the L-BPA uptake assay. BPA uptake lasted 5min and was measured at 4 hpi, after which fluorescence output was measured, the cells were lysed and total protein content was measured. Mean fluorescence data was normalized to the total protein content. Averages ± SEM of data from three biological repeats are shown for each condition. Statistical analysis was completed via one-way ANOVA Kruskal-Wallis test with Dunn’s multiple comparison and the respective p-values among the conditions compared are indicated.
Non Essential Amino Acid Solution, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/amino+acids/pm37418525-326-22-26?v=Beijing+Solarbio+Science
Average 95 stars, based on 1 article reviews
non essential amino acid solution - by Bioz Stars, 2026-08
95/100 stars
  Buy from Supplier
yeast nitrogen base w o amino acids  (Thermo Fisher)
95
Thermo Fisher yeast nitrogen base w o amino acids
(A) Schematic depiction of the LAT1-dependent uptake assay for import of the amino acid analog π-Boronophenylalanine <t>(L-BPA).</t> Cell were treated with L-BPA for 5min (1) after which cells were extensively washed, followed by the addition of a specific membrane permeable probe for additional 5 min (2), which emits fluorescent signal when bound to L-BPA(3). (B) Graph shows L- <t>BPA</t> <t>uptake</t> by Myd88 KO iBMDMs infected with the indicated Lp strains at MOI=20 or left uninfected (UN). In some control conditions, the LAT1 inhibitor 2-Aminobicyclo [2.2.1] heptane-2-carboxylic acid (BCH) at 1mM was added 10 min prior to and for the duration of the L-BPA uptake assay. BPA uptake lasted 5min and was measured at 4 hpi, after which fluorescence output was measured, the cells were lysed and total protein content was measured. Mean fluorescence data was normalized to the total protein content. Averages ± SEM of data from three biological repeats are shown for each condition. Statistical analysis was completed via one-way ANOVA Kruskal-Wallis test with Dunn’s multiple comparison and the respective p-values among the conditions compared are indicated.
Yeast Nitrogen Base W O Amino Acids, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/amino+acids/pmc03568079-161-32-42?v=Thermo+Fisher
Average 95 stars, based on 1 article reviews
yeast nitrogen base w o amino acids - by Bioz Stars, 2026-08
95/100 stars
  Buy from Supplier
3 bis  (Thermo Fisher)
94
Thermo Fisher 3 bis
(A) Schematic depiction of the LAT1-dependent uptake assay for import of the amino acid analog π-Boronophenylalanine <t>(L-BPA).</t> Cell were treated with L-BPA for 5min (1) after which cells were extensively washed, followed by the addition of a specific membrane permeable probe for additional 5 min (2), which emits fluorescent signal when bound to L-BPA(3). (B) Graph shows L- <t>BPA</t> <t>uptake</t> by Myd88 KO iBMDMs infected with the indicated Lp strains at MOI=20 or left uninfected (UN). In some control conditions, the LAT1 inhibitor 2-Aminobicyclo [2.2.1] heptane-2-carboxylic acid (BCH) at 1mM was added 10 min prior to and for the duration of the L-BPA uptake assay. BPA uptake lasted 5min and was measured at 4 hpi, after which fluorescence output was measured, the cells were lysed and total protein content was measured. Mean fluorescence data was normalized to the total protein content. Averages ± SEM of data from three biological repeats are shown for each condition. Statistical analysis was completed via one-way ANOVA Kruskal-Wallis test with Dunn’s multiple comparison and the respective p-values among the conditions compared are indicated.
3 Bis, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/amino+acids/10__1002_slash_er__7718-44-0-11?v=Thermo+Fisher
Average 94 stars, based on 1 article reviews
3 bis - by Bioz Stars, 2026-08
94/100 stars
  Buy from Supplier
2 amino 4 5 dimethoxybenzoic acid  (Thermo Fisher)
91
Thermo Fisher 2 amino 4 5 dimethoxybenzoic acid
(A) Schematic depiction of the LAT1-dependent uptake assay for import of the amino acid analog π-Boronophenylalanine <t>(L-BPA).</t> Cell were treated with L-BPA for 5min (1) after which cells were extensively washed, followed by the addition of a specific membrane permeable probe for additional 5 min (2), which emits fluorescent signal when bound to L-BPA(3). (B) Graph shows L- <t>BPA</t> <t>uptake</t> by Myd88 KO iBMDMs infected with the indicated Lp strains at MOI=20 or left uninfected (UN). In some control conditions, the LAT1 inhibitor 2-Aminobicyclo [2.2.1] heptane-2-carboxylic acid (BCH) at 1mM was added 10 min prior to and for the duration of the L-BPA uptake assay. BPA uptake lasted 5min and was measured at 4 hpi, after which fluorescence output was measured, the cells were lysed and total protein content was measured. Mean fluorescence data was normalized to the total protein content. Averages ± SEM of data from three biological repeats are shown for each condition. Statistical analysis was completed via one-way ANOVA Kruskal-Wallis test with Dunn’s multiple comparison and the respective p-values among the conditions compared are indicated.
2 Amino 4 5 Dimethoxybenzoic Acid, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/amino+acids/10__31549_slash_2542___1174___2023___7___3___27___36-66-6-13?v=Thermo+Fisher
Average 91 stars, based on 1 article reviews
2 amino 4 5 dimethoxybenzoic acid - by Bioz Stars, 2026-08
91/100 stars
  Buy from Supplier
fmoc amino ethoxy acetic acid  (Chem Impex International)
95
Chem Impex International fmoc amino ethoxy acetic acid
(A) Schematic depiction of the LAT1-dependent uptake assay for import of the amino acid analog π-Boronophenylalanine <t>(L-BPA).</t> Cell were treated with L-BPA for 5min (1) after which cells were extensively washed, followed by the addition of a specific membrane permeable probe for additional 5 min (2), which emits fluorescent signal when bound to L-BPA(3). (B) Graph shows L- <t>BPA</t> <t>uptake</t> by Myd88 KO iBMDMs infected with the indicated Lp strains at MOI=20 or left uninfected (UN). In some control conditions, the LAT1 inhibitor 2-Aminobicyclo [2.2.1] heptane-2-carboxylic acid (BCH) at 1mM was added 10 min prior to and for the duration of the L-BPA uptake assay. BPA uptake lasted 5min and was measured at 4 hpi, after which fluorescence output was measured, the cells were lysed and total protein content was measured. Mean fluorescence data was normalized to the total protein content. Averages ± SEM of data from three biological repeats are shown for each condition. Statistical analysis was completed via one-way ANOVA Kruskal-Wallis test with Dunn’s multiple comparison and the respective p-values among the conditions compared are indicated.
Fmoc Amino Ethoxy Acetic Acid, supplied by Chem Impex International, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/amino+acids/pmc12990034-334-16-23?v=Chem+Impex+International
Average 95 stars, based on 1 article reviews
fmoc amino ethoxy acetic acid - by Bioz Stars, 2026-08
95/100 stars
  Buy from Supplier
slc7a11  (Proteintech)
96
Proteintech slc7a11
(A) Schematic depiction of the LAT1-dependent uptake assay for import of the amino acid analog π-Boronophenylalanine <t>(L-BPA).</t> Cell were treated with L-BPA for 5min (1) after which cells were extensively washed, followed by the addition of a specific membrane permeable probe for additional 5 min (2), which emits fluorescent signal when bound to L-BPA(3). (B) Graph shows L- <t>BPA</t> <t>uptake</t> by Myd88 KO iBMDMs infected with the indicated Lp strains at MOI=20 or left uninfected (UN). In some control conditions, the LAT1 inhibitor 2-Aminobicyclo [2.2.1] heptane-2-carboxylic acid (BCH) at 1mM was added 10 min prior to and for the duration of the L-BPA uptake assay. BPA uptake lasted 5min and was measured at 4 hpi, after which fluorescence output was measured, the cells were lysed and total protein content was measured. Mean fluorescence data was normalized to the total protein content. Averages ± SEM of data from three biological repeats are shown for each condition. Statistical analysis was completed via one-way ANOVA Kruskal-Wallis test with Dunn’s multiple comparison and the respective p-values among the conditions compared are indicated.
Slc7a11, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/amino+acids/pm41872244-126-19-21?v=Proteintech
Average 96 stars, based on 1 article reviews
slc7a11 - by Bioz Stars, 2026-08
96/100 stars
  Buy from Supplier

Image Search Results


(A) Schematic depiction of the LAT1-dependent uptake assay for import of the amino acid analog π-Boronophenylalanine (L-BPA). Cell were treated with L-BPA for 5min (1) after which cells were extensively washed, followed by the addition of a specific membrane permeable probe for additional 5 min (2), which emits fluorescent signal when bound to L-BPA(3). (B) Graph shows L- BPA uptake by Myd88 KO iBMDMs infected with the indicated Lp strains at MOI=20 or left uninfected (UN). In some control conditions, the LAT1 inhibitor 2-Aminobicyclo [2.2.1] heptane-2-carboxylic acid (BCH) at 1mM was added 10 min prior to and for the duration of the L-BPA uptake assay. BPA uptake lasted 5min and was measured at 4 hpi, after which fluorescence output was measured, the cells were lysed and total protein content was measured. Mean fluorescence data was normalized to the total protein content. Averages ± SEM of data from three biological repeats are shown for each condition. Statistical analysis was completed via one-way ANOVA Kruskal-Wallis test with Dunn’s multiple comparison and the respective p-values among the conditions compared are indicated.

Journal: bioRxiv

Article Title: The Legionella pneumophila type IVb secretion system effector BinA subverts amino acid transport to sensitize TORC1 signaling in macrophages

doi: 10.1101/2025.02.20.639245

Figure Lengend Snippet: (A) Schematic depiction of the LAT1-dependent uptake assay for import of the amino acid analog π-Boronophenylalanine (L-BPA). Cell were treated with L-BPA for 5min (1) after which cells were extensively washed, followed by the addition of a specific membrane permeable probe for additional 5 min (2), which emits fluorescent signal when bound to L-BPA(3). (B) Graph shows L- BPA uptake by Myd88 KO iBMDMs infected with the indicated Lp strains at MOI=20 or left uninfected (UN). In some control conditions, the LAT1 inhibitor 2-Aminobicyclo [2.2.1] heptane-2-carboxylic acid (BCH) at 1mM was added 10 min prior to and for the duration of the L-BPA uptake assay. BPA uptake lasted 5min and was measured at 4 hpi, after which fluorescence output was measured, the cells were lysed and total protein content was measured. Mean fluorescence data was normalized to the total protein content. Averages ± SEM of data from three biological repeats are shown for each condition. Statistical analysis was completed via one-way ANOVA Kruskal-Wallis test with Dunn’s multiple comparison and the respective p-values among the conditions compared are indicated.

Article Snippet: BPA uptake assay (Dojindo Labratories, cat# UP04) was used to measure BPA transport in Lp-infected macrophages as per the suggested manufacturer’s protocol.

Techniques: Membrane, Infection, Control, Fluorescence, Comparison